제품 > 항체 > 다중클론항체(pAb)

QARS Rabbit pAb (A6960)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - QARS Rabbit pAb (A6960)

Western blot analysis of various lysates using QARS Rabbit pAb (A6960) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - QARS Rabbit pAb (A6960)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using QARS Rabbit pAb (A6960) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - QARS Rabbit pAb (A6960)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using QARS Rabbit pAb (A6960) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - QARS Rabbit pAb (A6960)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using QARS Rabbit pAb (A6960) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - QARS Rabbit pAb (A6960)

Immunofluorescence analysis of U2OS cells using QARS Rabbit pAb (A6960). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameQARS Rabbit pAb
Catalog No.A6960
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human QARS (NP_005042.1).
SequenceMAALDSLSLFTSLGLSEQKARETLKNSALSAQLREAATQAQQTLGSTIDKATGILLYGLASRLRDTRRLSFLVSYIASKKIHTEPQLSAALEYVRSHPLDPIDTVDFERECGVGVIVTPEQIEEAVEAAINRHRPQLLVERYHFNMGLLMGEARAVLKWADGKMIKNEVDMQVLHLLGPKLEADLEKKFKVAKARLEETDRRTAKDVVENGETADQTLSLMEQLRGEALKFHKPGENYKTPGYVVTPHTM
Gene ID
Swiss Prot
SynonymsQARS; GLNRS; MSCCA; PRO2195
Calculated MW88kDa
Observed MW88kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHL-60, 293T, HeLa, Mouse testis
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - QARS Rabbit pAb (A6960)}

    Western blot - QARS Rabbit pAb (A6960)

    Western blot analysis of various lysates using QARS Rabbit pAb (A6960) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - QARS Rabbit pAb (A6960)}

    Immunohistochemistry - QARS Rabbit pAb (A6960)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using QARS Rabbit pAb (A6960) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - QARS Rabbit pAb (A6960)}

    Immunohistochemistry - QARS Rabbit pAb (A6960)

    Immunohistochemistry analysis of paraffin-embedded Human liver damage using QARS Rabbit pAb (A6960) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - QARS Rabbit pAb (A6960)}

    Immunohistochemistry - QARS Rabbit pAb (A6960)

    Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using QARS Rabbit pAb (A6960) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - QARS Rabbit pAb (A6960)}

    Immunofluorescence - QARS Rabbit pAb (A6960)

    Immunofluorescence analysis of U2OS cells using QARS Rabbit pAb (A6960). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6960? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)