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RAB35 Rabbit pAb (A8030)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RAB35 Rabbit pAb (A8030)

Western blot analysis of various lysates using RAB35 Rabbit pAb (A8030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - RAB35 Rabbit pAb (A8030)

Western blot analysis of lysates from HeLa cells, using RAB35 Rabbit pAb (A8030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - RAB35 Rabbit pAb (A8030)

Immunohistochemistry analysis of paraffin-embedded Rat heart using RAB35 Rabbit pAb (A8030) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RAB35 Rabbit pAb (A8030)

Immunofluorescence analysis of PC-12 cells using RAB35 Rabbit pAb (A8030) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRAB35 Rabbit pAb
Catalog No.A8030
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables GTPase activity; guanyl ribonucleotide binding activity; and phosphatidylinositol-4, 5-bisphosphate binding activity. Involved in several processes, including endosomal transport; plasma membrane to endosome transport; and protein localization to endosome. Located in several cellular components, including clathrin-coated endocytic vesicle; clathrin-coated pit; and intercellular bridge.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-201 of human RAB35 (NP_006852.1).
SequenceKRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC
Gene ID
Swiss Prot
SynonymsRAY; H-ray; RAB1C; RAB35
Calculated MW23kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse brain, Rat brain
Cellular locationCell membrane, Cytoplasmic side, Cytoplasmic vesicle, Endosome, Lipid-anchor, Melanosome, Membrane, clathrin-coated pit, clathrin-coated vesicle
Customer validation

WB(Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - RAB35 Rabbit pAb (A8030)}

Western blot - RAB35 Rabbit pAb (A8030)

Western blot analysis of various lysates using RAB35 Rabbit pAb (A8030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - RAB35 Rabbit pAb (A8030)}

Western blot - RAB35 Rabbit pAb (A8030)

Western blot analysis of lysates from HeLa cells, using RAB35 Rabbit pAb (A8030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - RAB35 Rabbit pAb (A8030)}

Immunohistochemistry - RAB35 Rabbit pAb (A8030)

Immunohistochemistry analysis of paraffin-embedded Rat heart using RAB35 Rabbit pAb (A8030) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RAB35 Rabbit pAb (A8030)}

Immunofluorescence - RAB35 Rabbit pAb (A8030)

Immunofluorescence analysis of PC-12 cells using RAB35 Rabbit pAb (A8030) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A8030? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)