제품 > 항체 > 다중클론항체(pAb)

RABGAP1 Rabbit pAb (A15804)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - RABGAP1 Rabbit pAb (A15804)

Western blot analysis of various lysates using RABGAP1 Rabbit pAb (A15804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - RABGAP1 Rabbit pAb (A15804)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RABGAP1 Rabbit pAb (A15804) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RABGAP1 Rabbit pAb (A15804)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using RABGAP1 Rabbit pAb (A15804) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameRABGAP1 Rabbit pAb
Catalog No.A15804
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables GTPase activator activity and small GTPase binding activity. Involved in regulation of GTPase activity. Located in cytosol.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 830-1069 of human RABGAP1 (NP_036329.3).
SequenceQQEDPIERFERENRRLQEANMRLEQENDDLAHELVTSKIALRKDLDNAEEKADALNKELLMTKQKLIDAEEEKRRLEEESAQLKEMCRRELDKAESEIKKNSSIIGDYKQICSQLSERLEKQQTANKVEIEKIRQKVDDCERCREFFNKEGRVKGISSTKEVLDEDTDEEKETLKNQLREMELELAQTKLQLVEAECKIQDLEHHLGLALNEVQAAKKTWFNRTLSSIKTATGVQGKETC
Gene ID
Swiss Prot
SynonymsGAPCENA; TBC1D11; RABGAP1
Calculated MW122kDa
Observed MW122kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, HeLa, Mouse brain
Cellular locationCytoplasm, centrosome, cytoskeleton, cytosol, microtubule organizing center

Documents

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ABclonal:Western blot - RABGAP1 Rabbit pAb (A15804)}

Western blot - RABGAP1 Rabbit pAb (A15804)

Western blot analysis of various lysates using RABGAP1 Rabbit pAb (A15804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - RABGAP1 Rabbit pAb (A15804)}

Immunohistochemistry - RABGAP1 Rabbit pAb (A15804)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RABGAP1 Rabbit pAb (A15804) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RABGAP1 Rabbit pAb (A15804)}

Immunohistochemistry - RABGAP1 Rabbit pAb (A15804)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using RABGAP1 Rabbit pAb (A15804) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)