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RAMP2 Rabbit pAb (A3075)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RAMP2 Rabbit pAb (A3075)

Western blot analysis of various lysates using RAMP2 Rabbit pAb (A3075) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded mouse testis using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded rat heart using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameRAMP2 Rabbit pAb
Catalog No.A3075
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human RAMP2 (NP_005845.2).
SequenceMASLRVERAGGPRLPRTRVGRPAALRLLLLLGAVLNPHEALAQPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPIEKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFSDPPEDV
Gene ID
Swiss Prot
SynonymsRAMP2
Calculated MW20kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA-549, BxPC-3, MCF7
Cellular locationMembrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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ABclonal:Western blot - RAMP2 Rabbit pAb (A3075)}

Western blot - RAMP2 Rabbit pAb (A3075)

Western blot analysis of various lysates using RAMP2 Rabbit pAb (A3075) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)}

Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)}

Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)}

Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded mouse testis using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RAMP2 Rabbit pAb (A3075)}

Immunohistochemistry - RAMP2 Rabbit pAb (A3075)

Immunohistochemistry analysis of paraffin-embedded rat heart using RAMP2 Rabbit pAb (A3075) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)