제품 > 항체 > 다중클론항체(pAb)

RAP1B Rabbit pAb (A24472)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RAP1B Rabbit pAb (A24472)

Western blot analysis of lysates from Rat brain, using RAP1B Rabbit pAb (A24472) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameRAP1B Rabbit pAb
Catalog No.A24472
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 81-181 of human RAP1B (NP_056461.1).
SequenceVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSC
Gene ID
Swiss Prot
SynonymsRAP1B; K-REV; RAL1B; ras-related protein Rap-1b
Calculated MW15kDa/16kDa/18kDa/20kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationCell junction, Cell membrane, Cytoplasm, cytosol

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ABclonal:Western blot - RAP1B Rabbit pAb (A24472)}

Western blot - RAP1B Rabbit pAb (A24472)

Western blot analysis of lysates from Rat brain, using RAP1B Rabbit pAb (A24472) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

ELISA 키트 (1)

Secondary Antibodies (26)