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RASSF2 Rabbit mAb (A20904)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RASSF2 Rabbit mAb (A20904)

Western blot analysis of various lysates, using RASSF2 Rabbit mAb (A20904) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameRASSF2 Rabbit mAb
Catalog No.A20904
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2868
This gene encodes a protein that contains a Ras association domain. Similar to its cattle and sheep counterparts, this gene is located near the prion gene. Two alternatively spliced transcripts encoding the same isoform have been reported.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 227-326 of human RASSF2 (P50749).
SequenceHTSGEKQKLKATDYPLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKKLMRKYTVLRLMIRQRLEEIAETPATI
Gene ID
Swiss Prot
SynonymsCENP-34; RASFADIN; RASSF2
Calculated MW38kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHL-60, U-937, A-549, Mouse spleen, Mouse thymus, Rat thymus
Cellular locationCytoplasm, Nucleus

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ABclonal:Western blot - RASSF2 Rabbit mAb (A20904)}

Western blot - RASSF2 Rabbit mAb (A20904)

Western blot analysis of various lysates, using RASSF2 Rabbit mAb (A20904) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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