제품 > 항체 > 다중클론항체(pAb)

RAV1 Rabbit pAb (A21275)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - RAV1 Rabbit pAb (A21275)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using RAV1 Rabbit pAb (A21275) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameRAV1 Rabbit pAb
Catalog No.A21275
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Encodes an AP2/B3 domain transcription factor which is upregulated in response to low temperature. It contains a B3 DNA binding domain. It has circadian regulation and may function as a negative growth regulator.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 245-344 of arabidopsis thaliana RAV1 (NP_172784.1).
SequenceWNSSQSYVLTKGWSRFVKEKNLRAGDVVSFSRSNGQDQQLYIGWKSRSGSDLDAGRVLRLFGVNISPESSRNDVVGNKRVNDTEMLSLVCSKKQRIFHAS
Gene ID
Swiss Prot
SynonymsAtRAV1; EDF4; ETHYLENE RESPONSE DNA BINDING FACTOR 4; related to ABI3/VP1 1; T6J4.2; T6J4_2; RAV1
Calculated MW39kDa
Observed MW39kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSeedling, Inflorescences, Before inflorescence, Rosette leaf
Cellular locationnucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - RAV1 Rabbit pAb (A21275)}

    Western blot - RAV1 Rabbit pAb (A21275)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using RAV1 Rabbit pAb (A21275) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.