제품 > 항체 > 다중클론항체(pAb)

RBM15B Rabbit pAb (A9873)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - RBM15B Rabbit pAb (A9873)

Western blot analysis of extracts of various cell lines, using RBM15B Antibody (A9873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameRBM15B Rabbit pAb
Catalog No.A9873
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Members of the SPEN (Split-end) family of proteins, including RBM15B, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 540-720 of human RBM15B (NP_037418.3).
SequenceDRDRTFLEGDWTSPSKSSDRRNSLEGYSRSVRSRSGERWGADGDRGLPKPWEERRKRRSLSSDRGRTTHSPYEERSRTKGSGQQSERGSDRTPERSRKENHSSEGTKESSSNSLSNSRHGAEERGHHHHHHEAADSSHGKKARDSERNHRTTEAEPKPLEEPKHETKKLKNLSEYAQTLQL
Gene ID
Swiss Prot
SynonymsOTT3; HsOTT3; HUMAGCGB
Calculated MW97kDa
Observed MW97kDa/107kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, SH-SY5Y, HepG2, BT-474, Mouse pancreas, Mouse lung
Cellular locationNucleus, nucleoplasm

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ABclonal:Western blot - RBM15B Rabbit pAb (A9873)}

Western blot - RBM15B Rabbit pAb (A9873)

Western blot analysis of extracts of various cell lines, using RBM15B Antibody (A9873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)