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RBM15 Rabbit pAb (A4936)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using RBM15 Rabbit pAb (A4936) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - RBM15 Rabbit pAb (A4936)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RBM15 Rabbit pAb (A4936) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name	RBM15 Rabbit pAb
Catalog No.	A4936
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 530-780 of human RBM15 (NP_001188474.1).
Sequence	EHRYQQQYLQPLPLTHYELVTDAFGHRAPDPLRGARDRTPPLLYRDRDRDLYPDSDWVPPPPPVRERSTRTAATSVPAYEPLDSLDRRRDGWSLDRDRGDRDLPSSRDQPRKRRLPEESGGRHLDRSPESDRPRKRHCAPSPDRSPELSSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASA
Gene ID
Swiss Prot
Synonyms	OTT; OTT1; SPEN; RBM15
Calculated MW	107kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HL-60, HepG2
Cellular location	Nucleus, Nucleus membrane, Nucleus speckle, Peripheral membrane protein, nucleoplasm
Customer validation	WB(Homo sapiens) IF(Homo sapiens,Mus musculus) IHC(Homo sapiens,Mus musculus) WB(Homo sapiens,Mus musculus)