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RBM3 Rabbit mAb (A20911)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RBM3 Rabbit mAb (A20911)

Western blot analysis of various lysates using RBM3 Rabbit mAb (A20911) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RBM3 Rabbit mAb (A20911)

Immunofluorescence analysis of HeLa cells using RBM3 Rabbit mAb (A20911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RBM3 Rabbit mAb (A20911)

Immunofluorescence analysis of NIH/3T3 cells using RBM3 Rabbit mAb (A20911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RBM3 Rabbit mAb (A20911)

Immunofluorescence analysis of PC-12 cells using RBM3 Rabbit mAb (A20911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRBM3 Rabbit mAb
Catalog No.A20911
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2882
This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 58-157 of human RBM3 (P98179).
SequenceEHASVAMRAMNGESLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRYYDSRPGGYGYGYGRSRDYNGRNQGGYDRYSGGNYRDNYDN
Gene ID
Swiss Prot
SynonymsRNPL; IS1-RNPL; RBM3
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, Raji, RAW 264.7, C6, Neuro-2a, Rat thymus
Cellular locationCell projection, Cytoplasm, Nucleus, dendrite.

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ABclonal:Western blot - RBM3 Rabbit mAb (A20911)}

Western blot - RBM3 Rabbit mAb (A20911)

Western blot analysis of various lysates using RBM3 Rabbit mAb (A20911) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)}

Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)}

Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)}

Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RBM3 Rabbit mAb (A20911)}

Immunohistochemistry - RBM3 Rabbit mAb (A20911)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using RBM3 Rabbit mAb (A20911) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - RBM3 Rabbit mAb (A20911)}

Immunofluorescence - RBM3 Rabbit mAb (A20911)

Immunofluorescence analysis of HeLa cells using RBM3 Rabbit mAb (A20911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RBM3 Rabbit mAb (A20911)}

Immunofluorescence - RBM3 Rabbit mAb (A20911)

Immunofluorescence analysis of NIH/3T3 cells using RBM3 Rabbit mAb (A20911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RBM3 Rabbit mAb (A20911)}

Immunofluorescence - RBM3 Rabbit mAb (A20911)

Immunofluorescence analysis of PC-12 cells using RBM3 Rabbit mAb (A20911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)