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RBM3 Rabbit pAb (A5931)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RBM3 Rabbit pAb (A5931)

Western blot analysis of various lysates using RBM3 Rabbit pAb (A5931) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - RBM3 Rabbit pAb (A5931)

Immunohistochemistry analysis of paraffin-embedded Rat brain using RBM3 Rabbit pAb (A5931) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RBM3 Rabbit pAb (A5931)

Immunohistochemistry analysis of paraffin-embedded Human endometrium cancer using RBM3 Rabbit pAb (A5931) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RBM3 Rabbit pAb (A5931)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RBM3 Rabbit pAb (A5931) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameRBM3 Rabbit pAb
Catalog No.A5931
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human RBM3 (NP_006734.1).
SequenceMSSEEGKLFVGGLNFNTDEQALEDHFSSFGPISEVVVVKDRETQRSRGFGFITFTNPEHASVAMRAMNGESLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRYYDSRPGGYGYGYGRSRDYNGRNQGGYDRYSGGNYRDNYDN
Gene ID
Swiss Prot
SynonymsRNPL; IS1-RNPL; RBM3
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Jurkat, HL-60
Cellular locationCell projection, Cytoplasm, Nucleus, dendrite
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - RBM3 Rabbit pAb (A5931)}

Western blot - RBM3 Rabbit pAb (A5931)

Western blot analysis of various lysates using RBM3 Rabbit pAb (A5931) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - RBM3 Rabbit pAb (A5931)}

Immunohistochemistry - RBM3 Rabbit pAb (A5931)

Immunohistochemistry analysis of paraffin-embedded Rat brain using RBM3 Rabbit pAb (A5931) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RBM3 Rabbit pAb (A5931)}

Immunohistochemistry - RBM3 Rabbit pAb (A5931)

Immunohistochemistry analysis of paraffin-embedded Human endometrium cancer using RBM3 Rabbit pAb (A5931) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RBM3 Rabbit pAb (A5931)}

Immunohistochemistry - RBM3 Rabbit pAb (A5931)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RBM3 Rabbit pAb (A5931) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)