제품 > 항체 > 다중클론항체(pAb)

RCAN1 Rabbit pAb (A5326)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RCAN1 Rabbit pAb (A5326)

Western blot analysis of various lysates using RCAN1 Rabbit pAb (A5326) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

Immunohistochemistry analysis of paraffin-embedded Human brain using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

Immunohistochemistry analysis of paraffin-embedded Human liver using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

Immunohistochemistry analysis of paraffin-embedded Rat brain using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

Immunohistochemistry analysis of paraffin-embedded Rat heart using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameRCAN1 Rabbit pAb
Catalog No.A5326
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-245 of human RCAN1 (NP_004405.3).
SequenceDLQDLPSATIACHLDPRVFVDGLCRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPFSAADARLQLHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGPGEKYELHAATDTTPSVVVHVCESDQEKEEEEEMERMRRPKPKIIQTRRPE
Gene ID
Swiss Prot
SynonymsCSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78; RCAN1
Calculated MW28kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSW480, SKOV3, U-87MG
Cellular locationcytoplasm, nucleus
Customer validation

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - RCAN1 Rabbit pAb (A5326)}

    Western blot - RCAN1 Rabbit pAb (A5326)

    Western blot analysis of various lysates using RCAN1 Rabbit pAb (A5326) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)}

    Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

    Immunohistochemistry analysis of paraffin-embedded Human brain using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)}

    Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

    Immunohistochemistry analysis of paraffin-embedded Human liver using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)}

    Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)}

    Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

    Immunohistochemistry analysis of paraffin-embedded Mouse heart using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)}

    Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - RCAN1 Rabbit pAb (A5326)}

    Immunohistochemistry - RCAN1 Rabbit pAb (A5326)

    Immunohistochemistry analysis of paraffin-embedded Rat heart using RCAN1 Rabbit pAb (A5326) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A5326? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)