제품 > 항체 > 다중클론항체(pAb)

RGA1 Rabbit pAb (A19214)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - RGA1 Rabbit pAb (A19214)

Western blot analysis of various lysates using RGA1 Rabbit pAb (A19214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:8000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameRGA1 Rabbit pAb
Catalog No.A19214
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This? gene Ubiquitous expression in leaves before flowering (RPKM 48.7), leaves after flowering (RPKM 36.4) and 6 other tissues. It encodes protein Alpha subunit of G proteins (guanine nucleotide binding). G-alpha has three isomers, guanine nucleotide-binding protein alpha-1 subunit isoform X1,guanine nucleotide-binding protein alpha-1 subunit isoform X2,and guanine nucleotide-binding protein alpha-1 subunit isoform X3.RGA1 is one of the regulators in salt response partially through ROS scavenging, which might be helpful in elucidating salt tolerant mechanisms of heterotrimeric G protein in rice(PMID 30621186). The α-subunit of the rice heterotrimeric G protein, RGA1, regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1 (PMID 27194741).? The Gα heterotrimeric G protein subunit, RGA1, ? plays an important role in regulating photoprotection and photoprotection of rice (PMID 29216416).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-230 of Oryza sativa RGA1. (Q0DJ33).
SequenceSADIDRRILQETKAEQHIHKLLLLGAGESGKSTIFKQIKLLFQTGFDEAELRSYTSVIHANVYQTIKILYEGAKELSQVESDSSKYVISPDNQEIGEKLSDIDGRLDYPLLNKELVLDVKRLWQDPAIQETYLRGSILQLPDCAQYFMENLDRLAEAGYVPTKEDVLYARVRTNGVVQIQFSPVGENKRGGEVYRLYDVGGQRNERRKW
Gene ID
Swiss Prot
SynonymsD1; GA1; GPA1; RGA1; GP-alpha-1; OJ1005_D04.15
Calculated MW44kDa
Observed MW44.2kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRice root, Rice leaf
Cellular locationplasma membrane
    ABclonal:Western blot - RGA1 Rabbit pAb (A19214)}

    Western blot - RGA1 Rabbit pAb (A19214)

    Western blot analysis of various lysates using RGA1 Rabbit pAb (A19214) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:8000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)