제품 > 항체 > 다중클론항체(pAb)

RGMB Rabbit pAb (A12859)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RGMB Rabbit pAb (A12859)

Western blot analysis of various lysates using RGMB Rabbit pAb (A12859) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameRGMB Rabbit pAb
Catalog No.A12859
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
RGMB is a glycosylphosphatidylinositol (GPI)-anchored member of the repulsive guidance molecule family (see RGMA, MIM 607362) and contributes to the patterning of the developing nervous system (Samad et al., 2005 [PubMed 15671031]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-453 of human RGMB (NP_001012779.2).
SequencePHLRTFKDNFQTCKVEGAWPLIDNNYLSVQVTNVPVVPGSSATATNKITIIFKAHHECTDQKVYQAVTDDLPAAFVDGTTSGGDSDAKSLRIVERESGHYVEMHARYIGTTVFVRQVGRYLTLAIRMPEDLAMSYEESQDLQLCVNGCPLSERIDDGQGQVSAILGHSLPRTSLVQAWPGYTLETANTQCHEKMPVKDIYFQSCVFDLLTTGDANFTAAAHSALEDVEALHPRKERWHIFPSSG
Gene ID
Swiss Prot
SynonymsDRAGON; RGMB
Calculated MW48kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse brain, Rat brain
Cellular locationCell membrane, GPI-anchor, Lipid-anchor, Membrane raft
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - RGMB Rabbit pAb (A12859)}

    Western blot - RGMB Rabbit pAb (A12859)

    Western blot analysis of various lysates using RGMB Rabbit pAb (A12859) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A12859? Please let us know so that we can cite the reference in this datasheet.