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RHOG Rabbit pAb (A19302)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RHOG Rabbit pAb (A19302)

Western blot analysis of various lysates using RHOG Rabbit pAb (A19302) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - RHOG Rabbit pAb (A19302)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using RHOG Rabbit pAb (A19302) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RHOG Rabbit pAb (A19302)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using RHOG Rabbit pAb (A19302) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RHOG Rabbit pAb (A19302)

Immunohistochemistry analysis of paraffin-embedded human appendix using RHOG Rabbit pAb (A19302) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RHOG Rabbit pAb (A19302)

Immunofluorescence analysis of L929 cells using RHOG Rabbit pAb (A19302) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRHOG Rabbit pAb
Catalog No.A19302
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human RHOG (NP_001656.2).
SequenceTVNLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCPDVPILLVGTKKDLRAQPDTLRRLKEQGQAPITPQQGQALAKQIH
Gene ID
Swiss Prot
SynonymsARHG; RHOG
Calculated MW21kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationcell cortex, cell projection, cytoplasmic vesicle, cytoskeleton, cytosol, extracellular exosome, focal adhesion, plasma membrane

Documents

Certificate of Compliance

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ABclonal:Western blot - RHOG Rabbit pAb (A19302)}

Western blot - RHOG Rabbit pAb (A19302)

Western blot analysis of various lysates using RHOG Rabbit pAb (A19302) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - RHOG Rabbit pAb (A19302)}

Immunohistochemistry - RHOG Rabbit pAb (A19302)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using RHOG Rabbit pAb (A19302) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RHOG Rabbit pAb (A19302)}

Immunohistochemistry - RHOG Rabbit pAb (A19302)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using RHOG Rabbit pAb (A19302) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RHOG Rabbit pAb (A19302)}

Immunohistochemistry - RHOG Rabbit pAb (A19302)

Immunohistochemistry analysis of paraffin-embedded human appendix using RHOG Rabbit pAb (A19302) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RHOG Rabbit pAb (A19302)}

Immunofluorescence - RHOG Rabbit pAb (A19302)

Immunofluorescence analysis of L929 cells using RHOG Rabbit pAb (A19302) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)