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RIOX2/MINA53 Rabbit mAb (A19809)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RIOX2/MINA53 Rabbit mAb (A19809)

Western blot analysis of extracts of various cell lines, using RIOX2/RIOX2/MINA53 Rabbit mAb (A19809) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameRIOX2/MINA53 Rabbit mAb
Catalog No.A19809
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2336
MINA is a c-Myc (MYC; MIM 190080) target gene that may play a role in cell proliferation or regulation of cell growth. (Tsuneoka et al., 2002 [PubMed 12091391]; Zhang et al., 2005 [PubMed 15897898]).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 350-450 of human RIOX2/MINA53 (Q8IUF8).
SequenceLSTPGGKLPRLDSVVRLQFKDHIVLTVLPDQDQSDEAQEKMVYIYHSLKNSRETHMMGNEEETEFHGLRFPLSHLDALKQIWNSPAISVKDLKLTTDEEKE
Gene ID
Swiss Prot
SynonymsROX; MDIG; MINA; NO52; JMJD10; MINA53
Calculated MW53kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesBxPC-3, Mouse testis, Rat testis
Cellular locationcytosol, nucleolus, nucleoplasm

Documents

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    ABclonal:Western blot - RIOX2/MINA53 Rabbit mAb (A19809)}

    Western blot - RIOX2/MINA53 Rabbit mAb (A19809)

    Western blot analysis of extracts of various cell lines, using RIOX2/RIOX2/MINA53 Rabbit mAb (A19809) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)