제품 > 항체 > 다중클론항체(pAb)

RIPK3 Rabbit pAb (A12996)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using RIPK3 Rabbit pAb (A12996) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - RIPK3 Rabbit pAb (A12996)

Immunofluorescence analysis of Jurkat cells using RIPK3 Rabbit pAb (A12996) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264.7 cells using RIPK3 Rabbit pAb (A12996) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	RIPK3 Rabbit pAb
Catalog No.	A12996
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables NF-kappaB-inducing kinase activity and protein serine/threonine kinase activity. Involved in several processes, including execution phase of necroptosis; hematopoietic or lymphoid organ development; and regulation of programmed cell death. Acts upstream of or within several processes, including I-kappaB kinase/NF-kappaB signaling; positive regulation of intrinsic apoptotic signaling pathway; and positive regulation of protein deacetylation. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; hemolymphoid system gland; male reproductive gland or organ; primitive streak; and turbinate bone primordium. Orthologous to human RIPK3 (receptor interacting serine/threonine kinase 3).

Immunogen information

Immunogen	Recombinant protein of mouse RIPK3
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	Rip3; 2610528K09Rik; RIPK3
Calculated MW	46kDa
Observed MW	50-57kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HT-29, BxPC-3, NIH/3T3, Rat spleen
Cellular location	Cell membrane, Cytoplasm, Mitochondrion, cytosol
Customer validation	WB(Homo sapiens, Mus musculus)