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RNF10 Rabbit mAb (A25392)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RNF10 Rabbit mAb (A25392)

Western blot analysis of HeLa cells using RNF10 Rabbit mAb (A25392) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:60s.

ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)

Confocal imaging of PC-12 cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)

Confocal imaging of NIH/3T3 cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)

Confocal imaging of HeLa cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)

Confocal imaging of Hep G2 cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameRNF10 Rabbit mAb
Catalog No.A25392
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66424
The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 660-760 of human RNF10 (NP_055683.3).
SequenceSTEGHGALSISPLSRSPGSHADFLLTPLSPTASQGSPSFCVGSLEEDSPFPSFAQMLRVGKAKADVWPKTAPKKDENSLVPPAPVDSDGESDNSDRVPVPS
Gene ID
Swiss Prot
SynonymsRIE2
Calculated MW90kDa
Observed MW95kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - RNF10 Rabbit mAb (A25392)}

    Western blot - RNF10 Rabbit mAb (A25392)

    Western blot analysis of HeLa cells using RNF10 Rabbit mAb (A25392) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time:60s.
    ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)}

    Immunohistochemistry - RNF10 Rabbit mAb (A25392)

    Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)}

    Immunohistochemistry - RNF10 Rabbit mAb (A25392)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)}

    Immunohistochemistry - RNF10 Rabbit mAb (A25392)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RNF10 Rabbit mAb (A25392)}

    Immunohistochemistry - RNF10 Rabbit mAb (A25392)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using RNF10 Rabbit mAb (A25392) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)}

    Immunofluorescence - RNF10 Rabbit mAb (A25392)

    Confocal imaging of PC-12 cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)}

    Immunofluorescence - RNF10 Rabbit mAb (A25392)

    Confocal imaging of NIH/3T3 cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)}

    Immunofluorescence - RNF10 Rabbit mAb (A25392)

    Confocal imaging of HeLa cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - RNF10 Rabbit mAb (A25392)}

    Immunofluorescence - RNF10 Rabbit mAb (A25392)

    Confocal imaging of Hep G2 cells using RNF10 Rabbit mAb (A25392, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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