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RNF7 Rabbit pAb (A7301)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - RNF7 Rabbit pAb (A7301)

Immunofluorescence analysis of MCF-7 cells using RNF7 antibody (A7301). Blue: DAPI for nuclear staining.

Overview

Product nameRNF7 Rabbit pAb
Catalog No.A7301
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human RNF7 (NP_055060.1).
SequenceMADVEDGEETCALASHSGSSGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK
Gene ID
Swiss Prot
SynonymsSAG; ROC2; rbx2; CKBBP1
Calculated MW13kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples
Cellular locationCytoplasm, Nucleus

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ABclonal:Immunofluorescence - RNF7 Rabbit pAb (A7301)}

Immunofluorescence - RNF7 Rabbit pAb (A7301)

Immunofluorescence analysis of MCF-7 cells using RNF7 antibody (A7301). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)