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ROBO4 Rabbit pAb (A9876)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ROBO4 Rabbit pAb (A9876)

Western blot analysis of extracts of various cell lines, using ROBO4 antibody (A9876) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameROBO4 Rabbit pAb
Catalog No.A9876
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable cell-cell adhesion mediator activity. Involved in angiogenesis and establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 848-1007 of human ROBO4 (NP_061928.4).
SequenceGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS
Gene ID
Swiss Prot
SynonymsMRB; AOVD3; ECSM4
Calculated MW107kDa
Observed MW130kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, HepG2, HT-1080, HeLa
Cellular locationaxon, extracellular exosome, plasma membrane

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ABclonal:Western blot - ROBO4 Rabbit pAb (A9876)}

Western blot - ROBO4 Rabbit pAb (A9876)

Western blot analysis of extracts of various cell lines, using ROBO4 antibody (A9876) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

재조합 단백질 (1)

Secondary Antibodies (26)