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RPAP3 Rabbit pAb (A15239)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPAP3 Rabbit pAb (A15239)

Western blot analysis of various lysates using RPAP3 Rabbit pAb (A15239) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - RPAP3 Rabbit pAb (A15239)

Immunofluorescence analysis of HeLa cells using RPAP3 Rabbit pAb (A15239) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPAP3 Rabbit pAb (A15239)

Immunofluorescence analysis of L929 cells using RPAP3 Rabbit pAb (A15239) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPAP3 Rabbit pAb
Catalog No.A15239
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547).
SequenceNAEELQDFMRDLENWEKDIKQKDMELRRQNGVPEENLPPIRNGNFRKKKKGKAKESSKKTREENTKNRIKSYDYEAWAKLDVDRILDELDKDDSTHESLSQESESEEDGIHVDSQKALVLKEKGNKYFKQGKYDEAIDCYTKGMDADPYNPVLPTNRASAYFRLKKFAVAESDCNLAVALNRSYTKAYSRRGAARFALQKLEEAKKDYERVLELEPNNFEATNEL
Gene ID
Swiss Prot
SynonymsTah1; hSpagh; RPAP3
Calculated MW76kDa
Observed MW76kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Jurkat, 293T, HT-29, Rat testis
Cellular locationcytosol
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - RPAP3 Rabbit pAb (A15239)}

    Western blot - RPAP3 Rabbit pAb (A15239)

    Western blot analysis of various lysates using RPAP3 Rabbit pAb (A15239) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - RPAP3 Rabbit pAb (A15239)}

    Immunofluorescence - RPAP3 Rabbit pAb (A15239)

    Immunofluorescence analysis of HeLa cells using RPAP3 Rabbit pAb (A15239) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPAP3 Rabbit pAb (A15239)}

    Immunofluorescence - RPAP3 Rabbit pAb (A15239)

    Immunofluorescence analysis of L929 cells using RPAP3 Rabbit pAb (A15239) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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