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RPE65 Rabbit pAb (A25105)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - RPE65 Rabbit pAb (A25105)

Western blot analysis of lysates from Mouse eye using RPE65 Rabbit pAb(A25105) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:1s.

Overview

Product nameRPE65 Rabbit pAb
Catalog No.A25105
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital.
ImmunogenRecombinant Protein corresponding to a sequence within amino acids 340-440 of human RPE65(NP_000320.1).
SequenceYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLN
Gene ID
Swiss Prot
Synonymsp63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65; RPE65
Calculated MW61kDa
Observed MW66kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse eye
Cellular locationCell membrane, Cytoplasm, Lipid-anchor, Microsome membrane

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    ABclonal:Western blot - RPE65 Rabbit pAb (A25105)}

    Western blot - RPE65 Rabbit pAb (A25105)

    Western blot analysis of lysates from Mouse eye using RPE65 Rabbit pAb(A25105) at 1:1000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime:1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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