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RPE65 Rabbit mAb (A9615)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - RPE65 Rabbit mAb (A9615)

Western blot analysis of lysates from Rat eye, using RPE65 Rabbit mAb (A9615) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - RPE65 Rabbit mAb (A9615)

Immunohistochemistry analysis of paraffin-embedded Rat retina using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RPE65 Rabbit mAb (A9615)

Immunohistochemistry analysis of paraffin-embedded Mouse retina using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - RPE65 Rabbit mAb (A9615)

Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPE65 Rabbit mAb (A9615)

Immunofluorescence analysis of paraffin-embedded mouse eye using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPE65 Rabbit mAb
Catalog No.A9615
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1659
The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 400-500 of human RPE65 (Q16518).
SequenceTIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPA
Gene ID
Swiss Prot
Synonymsp63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65; RPE65
Calculated MW61kDa
Observed MW70kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:1000
  • IF/ICC 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat eye
Cellular locationCell membrane, Cytoplasm, Lipid-anchor, Microsome membrane.
Customer validation

WB(Mus musculus, Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - RPE65 Rabbit mAb (A9615)}

    Western blot - RPE65 Rabbit mAb (A9615)

    Western blot analysis of lysates from Rat eye, using RPE65 Rabbit mAb (A9615) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - RPE65 Rabbit mAb (A9615)}

    Immunohistochemistry - RPE65 Rabbit mAb (A9615)

    Immunohistochemistry analysis of paraffin-embedded Rat retina using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RPE65 Rabbit mAb (A9615)}

    Immunohistochemistry - RPE65 Rabbit mAb (A9615)

    Immunohistochemistry analysis of paraffin-embedded Mouse retina using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - RPE65 Rabbit mAb (A9615)}

    Immunofluorescence - RPE65 Rabbit mAb (A9615)

    Immunofluorescence analysis of paraffin-embedded rat eye using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPE65 Rabbit mAb (A9615)}

    Immunofluorescence - RPE65 Rabbit mAb (A9615)

    Immunofluorescence analysis of paraffin-embedded mouse eye using RPE65 Rabbit mAb (A9615) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9615? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (26)