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RPL10A Rabbit pAb (A5925)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPL10A Rabbit pAb (A5925)

Western blot analysis of various lysates using RPL10A Rabbit pAb (A5925) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - RPL10A Rabbit pAb (A5925)

Immunohistochemistry analysis of paraffin-embedded Rat cerebellum using RPL10A Rabbit pAb (A5925) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL10A Rabbit pAb (A5925)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPL10A Rabbit pAb (A5925) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RPL10A Rabbit pAb (A5925)

Immunofluorescence analysis of C6 cells using RPL10A Rabbit pAb (A5925) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPL10A Rabbit pAb
Catalog No.A5925
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human RPL10A (NP_009035.3).
SequenceMSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKLVKKLAKKYDAF
Gene ID
Swiss Prot
SynonymsuL1; L10A; CSA19; NEDD6; Csa-19; RPL10A
Calculated MW25kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSKOV3, U-937, Mouse spleen, Rat spleen, Rat uterus
Cellular locationcytoplasm, cytosol, cytosolic ribosome, extracellular exosome, focal adhesion, nucleus

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ABclonal:Western blot - RPL10A Rabbit pAb (A5925)}

Western blot - RPL10A Rabbit pAb (A5925)

Western blot analysis of various lysates using RPL10A Rabbit pAb (A5925) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - RPL10A Rabbit pAb (A5925)}

Immunohistochemistry - RPL10A Rabbit pAb (A5925)

Immunohistochemistry analysis of paraffin-embedded Rat cerebellum using RPL10A Rabbit pAb (A5925) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL10A Rabbit pAb (A5925)}

Immunohistochemistry - RPL10A Rabbit pAb (A5925)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPL10A Rabbit pAb (A5925) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RPL10A Rabbit pAb (A5925)}

Immunofluorescence - RPL10A Rabbit pAb (A5925)

Immunofluorescence analysis of C6 cells using RPL10A Rabbit pAb (A5925) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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