제품 > 항체 > 다중클론항체(pAb)

RPL17 Rabbit pAb (A5934)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPL17 Rabbit pAb (A5934)

Western blot analysis of various lysates using RPL17 Rabbit pAb (A5934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - RPL17 Rabbit pAb (A5934)

Immunohistochemistry analysis of paraffin-embedded Rat brain using RPL17 Rabbit pAb (A5934) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL17 Rabbit pAb (A5934)

Immunohistochemistry analysis of paraffin-embedded Human placenta using RPL17 Rabbit pAb (A5934) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL17 Rabbit pAb (A5934)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPL17 Rabbit pAb (A5934) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of C6 cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of HeLa cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of L929 cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of U-2 OS cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPL17 Rabbit pAb
Catalog No.A5934
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RPL17 (NP_000976.1).
SequenceMVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLQKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKISQKKLKKQKLMARE
Gene ID
Swiss Prot
SynonymsL17; PD-1; uL22; RPL23; RPL17
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, 293T, A-549, SW480, HeLa, Mouse lung, Mouse thymus, Rat liver
Cellular locationcytoplasm, cytosol, cytosolic ribosome, nucleus

Documents

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ABclonal:Western blot - RPL17 Rabbit pAb (A5934)}

Western blot - RPL17 Rabbit pAb (A5934)

Western blot analysis of various lysates using RPL17 Rabbit pAb (A5934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - RPL17 Rabbit pAb (A5934)}

Immunohistochemistry - RPL17 Rabbit pAb (A5934)

Immunohistochemistry analysis of paraffin-embedded Rat brain using RPL17 Rabbit pAb (A5934) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL17 Rabbit pAb (A5934)}

Immunohistochemistry - RPL17 Rabbit pAb (A5934)

Immunohistochemistry analysis of paraffin-embedded Human placenta using RPL17 Rabbit pAb (A5934) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL17 Rabbit pAb (A5934)}

Immunohistochemistry - RPL17 Rabbit pAb (A5934)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPL17 Rabbit pAb (A5934) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)}

Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of C6 cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)}

Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of HeLa cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)}

Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of L929 cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPL17 Rabbit pAb (A5934)}

Immunofluorescence - RPL17 Rabbit pAb (A5934)

Immunofluorescence analysis of U-2 OS cells using RPL17 Rabbit pAb (A5934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)