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RPL36AL Rabbit pAb (A14366)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPL36AL Rabbit pAb (A14366)

Western blot analysis of various lysates using RPL36AL Rabbit pAb (A14366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)

Immunofluorescence analysis of C6 cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)

Immunofluorescence analysis of L929 cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)

Immunofluorescence analysis of U-2 OS cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)

Confocal immunofluorescence analysis of HeLa cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameRPL36AL Rabbit pAb
Catalog No.A14366
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which shares sequence similarity with yeast ribosomal protein L44, belongs to the L44E (L36AE) family of ribosomal proteins. This gene and the human gene officially named ribosomal protein L36a (RPL36A) encode nearly identical proteins; however, they are distinct genes. Although the name of this gene has been referred to as ribosomal protein L36a (RPL36A), its official name is ribosomal protein L36a-like (RPL36AL). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human RPL36AL (NP_000992.1).
SequenceMVNVPKTRRTFCKKCGKHQPHKVTQYKKGKDSLYAQGRRRYDRKQSGYGGQTKPIFRKKAKTTKKIVLRLECVEPNCRSKRMLAIKRCKHFELGGDKKRKGQVIQF
Gene ID
Swiss Prot
SynonymsRPL36A; RPL36AP42; RPL36A_18_1363; RPL36AL
Calculated MW12kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, A-549, HeLa, Jurkat, Mouse kidney, Mouse lung
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - RPL36AL Rabbit pAb (A14366)}

    Western blot - RPL36AL Rabbit pAb (A14366)

    Western blot analysis of various lysates using RPL36AL Rabbit pAb (A14366) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)}

    Immunofluorescence - RPL36AL Rabbit pAb (A14366)

    Immunofluorescence analysis of C6 cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)}

    Immunofluorescence - RPL36AL Rabbit pAb (A14366)

    Immunofluorescence analysis of L929 cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)}

    Immunofluorescence - RPL36AL Rabbit pAb (A14366)

    Immunofluorescence analysis of U-2 OS cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPL36AL Rabbit pAb (A14366)}

    Immunofluorescence - RPL36AL Rabbit pAb (A14366)

    Confocal immunofluorescence analysis of HeLa cells using RPL36AL Rabbit pAb (A14366) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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