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RPL4 Rabbit pAb (A5886)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPL4 Rabbit pAb (A5886)

Western blot analysis of various lysates using RPL4 Rabbit pAb (A5886) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - RPL4 Rabbit pAb (A5886)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using RPL4 Rabbit pAb (A5886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL4 Rabbit pAb (A5886)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPL4 Rabbit pAb (A5886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameRPL4 Rabbit pAb
Catalog No.A5886
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPL4 (NP_000959.2).
SequenceMACARPLISVYSEKGESSGKNVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKL
Gene ID
Swiss Prot
SynonymsL4; uL4; RPL4
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, HeLa, HepG2, HL-60, HT-1080, MCF7, Mouse liver
Cellular locationcytoplasm, cytosol, cytosolic ribosome, extracellular exosome, focal adhesion, nucleolus, nucleus, rough endoplasmic reticulum

Documents

Certificate of Compliance

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ABclonal:Western blot - RPL4 Rabbit pAb (A5886)}

Western blot - RPL4 Rabbit pAb (A5886)

Western blot analysis of various lysates using RPL4 Rabbit pAb (A5886) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - RPL4 Rabbit pAb (A5886)}

Immunohistochemistry - RPL4 Rabbit pAb (A5886)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using RPL4 Rabbit pAb (A5886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL4 Rabbit pAb (A5886)}

Immunohistochemistry - RPL4 Rabbit pAb (A5886)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPL4 Rabbit pAb (A5886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)