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RPLP1 Rabbit pAb (A6725)

Review (1)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Zebrafish

ABclonal:Western blot - RPLP1 Rabbit pAb (A6725)

Western blot analysis of various lysates using RPLP1 Rabbit pAb (A6725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - RPLP1 Rabbit pAb (A6725)

Immunofluorescence analysis of MCF7 cells using RPLP1 Rabbit pAb (A6725). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for RPLP1 Rabbit pAb(A6725)

Overview

Product nameRPLP1 Rabbit pAb
Catalog No.A6725
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human RPLP1 (NP_000994.1).
SequenceMASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD
Gene ID
Swiss Prot
SynonymsP1; LP1; RPP1; RPLP1
Calculated MW12kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Zebrafish
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSW480, Mouse spleen, Mouse lung, Mouse brain, Rat spleen, Rat lung, Rat brain
Cellular locationcytoplasm, cytosol, cytosolic ribosome, focal adhesion
Customer validation

(Zebrafish)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - RPLP1 Rabbit pAb (A6725)}

    Western blot - RPLP1 Rabbit pAb (A6725)

    Western blot analysis of various lysates using RPLP1 Rabbit pAb (A6725) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - RPLP1 Rabbit pAb (A6725)}

    Immunofluorescence - RPLP1 Rabbit pAb (A6725)

    Immunofluorescence analysis of MCF7 cells using RPLP1 Rabbit pAb (A6725). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)