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RPLP2 Rabbit pAb (A6974)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - RPLP2 Rabbit pAb (A6974)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RPLP2 Rabbit pAb (A6974) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RPLP2 Rabbit pAb (A6974)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using RPLP2 Rabbit pAb (A6974) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RPLP2 Rabbit pAb (A6974)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using RPLP2 Rabbit pAb (A6974) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RPLP2 Rabbit pAb (A6974)

Immunofluorescence analysis of U2OS cells using RPLP2 Rabbit pAb (A6974). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPLP2 Rabbit pAb
Catalog No.A6974
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P1. The P2 protein can interact with P0 and P1 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human RPLP2 (NP_000995.1).
SequenceMRYVASYLLAALGGNSSPSAKDIKKILDSVGIEADDDRLNKVISELNGKNIEDVIAQGIGKLASVPAGGAVAVSAAPGSAAPAAGSAPAAAEEKKDEKKEESEESDDDMGFGLFD
Gene ID
Swiss Prot
SynonymsP2; LP2; RPP2; D11S2243E; RPLP2
Calculated MW12kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationcytoplasm, cytosol, extracellular exosome, focal adhesion.

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ABclonal:Immunohistochemistry - RPLP2 Rabbit pAb (A6974)}

Immunohistochemistry - RPLP2 Rabbit pAb (A6974)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RPLP2 Rabbit pAb (A6974) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RPLP2 Rabbit pAb (A6974)}

Immunohistochemistry - RPLP2 Rabbit pAb (A6974)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using RPLP2 Rabbit pAb (A6974) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RPLP2 Rabbit pAb (A6974)}

Immunohistochemistry - RPLP2 Rabbit pAb (A6974)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using RPLP2 Rabbit pAb (A6974) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - RPLP2 Rabbit pAb (A6974)}

Immunofluorescence - RPLP2 Rabbit pAb (A6974)

Immunofluorescence analysis of U2OS cells using RPLP2 Rabbit pAb (A6974). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)