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RPN1 Rabbit pAb (A12497)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPN1 Rabbit pAb (A12497)

Western blot analysis of various lysates using RPN1 Rabbit pAb (A12497) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - RPN1 Rabbit pAb (A12497)

Immunohistochemistry analysis of paraffin-embedded rat kidney using RPN1 Rabbit pAb (A12497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPN1 Rabbit pAb (A12497)

Immunohistochemistry analysis of paraffin-embedded human tonsil using RPN1 Rabbit pAb (A12497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPN1 Rabbit pAb (A12497)

Immunohistochemistry analysis of paraffin-embedded mouse brain using RPN1 Rabbit pAb (A12497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameRPN1 Rabbit pAb
Catalog No.A12497
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
SequenceAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSF
Gene ID
Swiss Prot
SynonymsOST1; RBPH1; RPN1
Calculated MW69kDa
Observed MW68kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHL-60, HepG2, SKOV3, MCF7, BxPC-3, Mouse liver, Rat liver
Cellular locationEndoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - RPN1 Rabbit pAb (A12497)}

Western blot - RPN1 Rabbit pAb (A12497)

Western blot analysis of various lysates using RPN1 Rabbit pAb (A12497) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - RPN1 Rabbit pAb (A12497)}

Immunohistochemistry - RPN1 Rabbit pAb (A12497)

Immunohistochemistry analysis of paraffin-embedded rat kidney using RPN1 Rabbit pAb (A12497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPN1 Rabbit pAb (A12497)}

Immunohistochemistry - RPN1 Rabbit pAb (A12497)

Immunohistochemistry analysis of paraffin-embedded human tonsil using RPN1 Rabbit pAb (A12497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPN1 Rabbit pAb (A12497)}

Immunohistochemistry - RPN1 Rabbit pAb (A12497)

Immunohistochemistry analysis of paraffin-embedded mouse brain using RPN1 Rabbit pAb (A12497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12497? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)