제품 > 항체 > 다중클론항체(pAb)

RPP25 Rabbit pAb (A9973)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - RPP25 Rabbit pAb (A9973)

Western blot analysis of various lysates using RPP25 Rabbit pAb (A9973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - RPP25 Rabbit pAb (A9973)

Immunohistochemistry analysis of paraffin-embedded Human colon using RPP25 Rabbit pAb (A9973) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPP25 Rabbit pAb (A9973)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPP25 Rabbit pAb (A9973) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameRPP25 Rabbit pAb
Catalog No.A9973
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ribonuclease P RNA binding activity. Contributes to ribonuclease P activity. Involved in tRNA 5'-leader removal. Located in centriolar satellite and nucleoplasm. Part of multimeric ribonuclease P complex and ribonuclease MRP complex. Biomarker of autistic disorder.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human RPP25 (NP_060263.2).
SequenceMENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQPATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEPGVADEDQTA
Gene ID
Swiss Prot
SynonymsRPP25
Calculated MW21kDa
Observed MW20kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA375, HeLa, HT-29
Cellular locationNucleus

Documents

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ABclonal:Western blot - RPP25 Rabbit pAb (A9973)}

Western blot - RPP25 Rabbit pAb (A9973)

Western blot analysis of various lysates using RPP25 Rabbit pAb (A9973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - RPP25 Rabbit pAb (A9973)}

Immunohistochemistry - RPP25 Rabbit pAb (A9973)

Immunohistochemistry analysis of paraffin-embedded Human colon using RPP25 Rabbit pAb (A9973) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPP25 Rabbit pAb (A9973)}

Immunohistochemistry - RPP25 Rabbit pAb (A9973)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using RPP25 Rabbit pAb (A9973) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)