제품 > 항체 > 다중클론항체(pAb)

RPS10 Rabbit pAb (A5880)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS10 Rabbit pAb (A5880)

Western blot analysis of various lysates using RPS10 Rabbit pAb (A5880) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 30s.

ABclonal:Western blot - RPS10 Rabbit pAb (A5880)

Western blot analysis of lysates from Rat thymus using RPS10 Rabbit pAb(A5880) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:180s.

ABclonal:Immunohistochemistry - RPS10 Rabbit pAb (A5880)

Immunohistochemistry analysis of RPS10 in paraffin-embedded human skin tissue using RPS10 Rabbit pAb (A5880) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RPS10 Rabbit pAb (A5880)

Immunohistochemistry analysis of RPS10 in paraffin-embedded mouse kidney tissue using RPS10 Rabbit pAb (A5880) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RPS10 Rabbit pAb (A5880)

Immunohistochemistry analysis of RPS10 in paraffin-embedded rat colon tissue using RPS10 Rabbit pAb (A5880) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RPS10 Rabbit pAb (A5880)

Immunofluorescence analysis of NIH/3T3 cells using RPS10 Rabbit pAb(A5880) at a dilution of 1:500 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - RPS10 Rabbit pAb (A5880)

Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells using 1 μg RPS10 antibody (A5880). Western blot was performed from the immunoprecipitate using RPS10 antibody (A5880) at a dilution of 1:1000.

Overview

Product nameRPS10 Rabbit pAb
Catalog No.A5880
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPS10 (NP_001190174.1).
SequenceMLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ
Gene ID
Swiss Prot
SynonymsS10; DBA9; eS10; RPS10
Calculated MW19kDa
Observed MW19kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:500
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, K-562, Mouse liver, Rat thymus
Cellular locationCytoplasm, Nucleus, nucleolus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - RPS10 Rabbit pAb (A5880)}

Western blot - RPS10 Rabbit pAb (A5880)

Western blot analysis of various lysates using RPS10 Rabbit pAb (A5880) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 30s.
ABclonal:Western blot - RPS10 Rabbit pAb (A5880)}

Western blot - RPS10 Rabbit pAb (A5880)

Western blot analysis of lysates from Rat thymus using RPS10 Rabbit pAb(A5880) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:180s.
ABclonal:Immunohistochemistry - RPS10 Rabbit pAb (A5880)}

Immunohistochemistry - RPS10 Rabbit pAb (A5880)

Immunohistochemistry analysis of RPS10 in paraffin-embedded human skin tissue using RPS10 Rabbit pAb (A5880) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RPS10 Rabbit pAb (A5880)}

Immunohistochemistry - RPS10 Rabbit pAb (A5880)

Immunohistochemistry analysis of RPS10 in paraffin-embedded mouse kidney tissue using RPS10 Rabbit pAb (A5880) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RPS10 Rabbit pAb (A5880)}

Immunohistochemistry - RPS10 Rabbit pAb (A5880)

Immunohistochemistry analysis of RPS10 in paraffin-embedded rat colon tissue using RPS10 Rabbit pAb (A5880) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - RPS10 Rabbit pAb (A5880)}

Immunofluorescence - RPS10 Rabbit pAb (A5880)

Immunofluorescence analysis of NIH/3T3 cells using RPS10 Rabbit pAb(A5880) at a dilution of 1:500 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - RPS10 Rabbit pAb (A5880)}

Immunoprecipitation - RPS10 Rabbit pAb (A5880)

Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells using 1 μg RPS10 antibody (A5880). Western blot was performed from the immunoprecipitate using RPS10 antibody (A5880) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)