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RPS13 Rabbit pAb (A15720)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS13 Rabbit pAb (A15720)

Western blot analysis of various lysates using RPS13 Rabbit pAb (A15720) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - RPS13 Rabbit pAb (A15720)

Immunohistochemistry analysis of paraffin-embedded human placenta using RPS13 Rabbit pAb (A15720) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RPS13 Rabbit pAb (A15720)

Immunofluorescence analysis of L929 cells using RPS13 Rabbit pAb (A15720) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPS13 Rabbit pAb (A15720)

Immunofluorescence analysis of U-2 OS cells using RPS13 Rabbit pAb (A15720) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPS13 Rabbit pAb
Catalog No.A15720
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 79-151 of human RPS13 (NP_001008.1).
SequenceGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA
Gene ID
Swiss Prot
SynonymsS13; uS15; RPS13
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, U-87MG, BxPC-3, LO2, Mouse uterus, Mouse liver, Mouse brain, Rat liver
Cellular locationcytoplasm, cytosol, cytosolic ribosome, extracellular exosome, focal adhesion, nucleolus, nucleoplasm, nucleus
Customer validation

WB(Eriocheir sinensis H.Milne-Edwards, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - RPS13 Rabbit pAb (A15720)}

    Western blot - RPS13 Rabbit pAb (A15720)

    Western blot analysis of various lysates using RPS13 Rabbit pAb (A15720) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunohistochemistry - RPS13 Rabbit pAb (A15720)}

    Immunohistochemistry - RPS13 Rabbit pAb (A15720)

    Immunohistochemistry analysis of paraffin-embedded human placenta using RPS13 Rabbit pAb (A15720) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - RPS13 Rabbit pAb (A15720)}

    Immunofluorescence - RPS13 Rabbit pAb (A15720)

    Immunofluorescence analysis of L929 cells using RPS13 Rabbit pAb (A15720) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPS13 Rabbit pAb (A15720)}

    Immunofluorescence - RPS13 Rabbit pAb (A15720)

    Immunofluorescence analysis of U-2 OS cells using RPS13 Rabbit pAb (A15720) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15720? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)