제품 > 항체 > 다중클론항체(pAb)

RPS18 Rabbit pAb (A11687)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS18 Rabbit pAb (A11687)

Western blot analysis of various lysates using RPS18 Rabbit pAb (A11687) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RPS18 Rabbit pAb (A11687)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RPS18 Rabbit pAb (A11687) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RPS18 Rabbit pAb (A11687)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using RPS18 Rabbit pAb (A11687) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RPS18 Rabbit pAb (A11687)

Immunohistochemistry analysis of paraffin-embedded Rat brain using RPS18 Rabbit pAb (A11687) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)

Immunofluorescence analysis of HepG2 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)

Immunofluorescence analysis of NIH/3T3 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)

Immunofluorescence analysis of PC-12 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)

Immunofluorescence analysis of THP-1 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPS18 Rabbit pAb
Catalog No.A11687
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human RPS18 (NP_072045.1).
SequenceMSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK
Gene ID
Swiss Prot
SynonymsKE3; S18; HKE3; KE-3; uS13; D6S218E; RPS18
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, Mouse liver, Mouse thymus, Rat liver
Cellular locationCytoplasm.
Customer validation

WB(Homo sapiens, Mus musculus, Eriocheir sinensis H.Milne-Edwards)

DB(Murrah bufalo)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - RPS18 Rabbit pAb (A11687)}

    Western blot - RPS18 Rabbit pAb (A11687)

    Western blot analysis of various lysates using RPS18 Rabbit pAb (A11687) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - RPS18 Rabbit pAb (A11687)}

    Immunohistochemistry - RPS18 Rabbit pAb (A11687)

    Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RPS18 Rabbit pAb (A11687) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RPS18 Rabbit pAb (A11687)}

    Immunohistochemistry - RPS18 Rabbit pAb (A11687)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using RPS18 Rabbit pAb (A11687) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RPS18 Rabbit pAb (A11687)}

    Immunohistochemistry - RPS18 Rabbit pAb (A11687)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using RPS18 Rabbit pAb (A11687) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)}

    Immunofluorescence - RPS18 Rabbit pAb (A11687)

    Immunofluorescence analysis of HepG2 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)}

    Immunofluorescence - RPS18 Rabbit pAb (A11687)

    Immunofluorescence analysis of NIH/3T3 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)}

    Immunofluorescence - RPS18 Rabbit pAb (A11687)

    Immunofluorescence analysis of PC-12 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RPS18 Rabbit pAb (A11687)}

    Immunofluorescence - RPS18 Rabbit pAb (A11687)

    Immunofluorescence analysis of THP-1 cells using RPS18 Rabbit pAb (A11687) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A11687? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)