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RPS19BP1 Rabbit pAb (A13231)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS19BP1 Rabbit pAb (A13231)

Western blot analysis of various lysates using RPS19BP1 Rabbit pAb (A13231) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - RPS19BP1 Rabbit pAb (A13231)

Immunofluorescence analysis of L929 cells using RPS19BP1 Rabbit pAb (A13231) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPS19BP1 Rabbit pAb
Catalog No.A13231
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables enzyme binding activity. Located in cytosol; nucleolus; and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human RPS19BP1 (NP_919307.1).
SequenceMSAALLRRGLELLAASEAPRDPPGQAKPRGAPVKRPRKTKAIQAQKLRNSAKGKVPKSALDEYRKRECRDHLRVNLKFLTRTRSTVAESVSQQILRQNRGRKACDRPVAKTKKKKAEGTVFTEEDFQKFQQEYFGS
Gene ID
Swiss Prot
SynonymsAROS; S19BP; RPS19BP1
Calculated MW15kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-549, A-431, Jurkat, MCF7
Cellular locationNucleus, nucleolus
Customer validation

IF(Homo sapiens)

WB(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - RPS19BP1 Rabbit pAb (A13231)}

Western blot - RPS19BP1 Rabbit pAb (A13231)

Western blot analysis of various lysates using RPS19BP1 Rabbit pAb (A13231) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - RPS19BP1 Rabbit pAb (A13231)}

Immunofluorescence - RPS19BP1 Rabbit pAb (A13231)

Immunofluorescence analysis of L929 cells using RPS19BP1 Rabbit pAb (A13231) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)