제품 > 항체 > 다중클론항체(pAb)

RPS19BP1 Rabbit pAb (A13231)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using RPS19BP1 Rabbit pAb (A13231) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - RPS19BP1 Rabbit pAb (A13231)

Immunofluorescence analysis of L929 cells using RPS19BP1 Rabbit pAb (A13231) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	RPS19BP1 Rabbit pAb
Catalog No.	A13231
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables enzyme binding activity. Located in cytosol; nucleolus; and nucleoplasm.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human RPS19BP1 (NP_919307.1).
Sequence	MSAALLRRGLELLAASEAPRDPPGQAKPRGAPVKRPRKTKAIQAQKLRNSAKGKVPKSALDEYRKRECRDHLRVNLKFLTRTRSTVAESVSQQILRQNRGRKACDRPVAKTKKKKAEGTVFTEEDFQKFQQEYFGS
Gene ID
Swiss Prot
Synonyms	AROS; S19BP; RPS19BP1
Calculated MW	15kDa
Observed MW	20kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	A-549, A-431, Jurkat, MCF7
Cellular location	Nucleus, nucleolus
Customer validation	IF(Homo sapiens) WB(Homo sapiens) WB(Homo sapiens)