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RPS27A Rabbit pAb (A2027)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS27A Rabbit pAb (A2027)

Western blot analysis of lysates from 293F cells using RPS27A Rabbit pAb(A2027) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - RPS27A Rabbit pAb (A2027)

Immunohistochemistry analysis of paraffin-embedded rat kidney using RPS27A Rabbit pAb (A2027) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPS27A Rabbit pAb (A2027)

Immunohistochemistry analysis of paraffin-embedded human placenta using RPS27A Rabbit pAb (A2027) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPS27A Rabbit pAb (A2027)

Immunohistochemistry analysis of paraffin-embedded mouse brain using RPS27A Rabbit pAb (A2027) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RPS27A Rabbit pAb (A2027)

Immunofluorescence analysis of MCF7 cells using RPS27A Rabbit pAb (A2027) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRPS27A Rabbit pAb
Catalog No.A2027
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPS27A (NP_001170884.1).
SequenceMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK
Gene ID
Swiss Prot
SynonymsUBC; S27A; eS31; CEP80; UBA80; HEL112; UBCEP1; UBCEP80; RPS27A
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293F
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

IF(Mus musculus, Homo sapiens)

IF(Homo sapiens, Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - RPS27A Rabbit pAb (A2027)}

Western blot - RPS27A Rabbit pAb (A2027)

Western blot analysis of lysates from 293F cells using RPS27A Rabbit pAb(A2027) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - RPS27A Rabbit pAb (A2027)}

Immunohistochemistry - RPS27A Rabbit pAb (A2027)

Immunohistochemistry analysis of paraffin-embedded rat kidney using RPS27A Rabbit pAb (A2027) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPS27A Rabbit pAb (A2027)}

Immunohistochemistry - RPS27A Rabbit pAb (A2027)

Immunohistochemistry analysis of paraffin-embedded human placenta using RPS27A Rabbit pAb (A2027) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPS27A Rabbit pAb (A2027)}

Immunohistochemistry - RPS27A Rabbit pAb (A2027)

Immunohistochemistry analysis of paraffin-embedded mouse brain using RPS27A Rabbit pAb (A2027) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RPS27A Rabbit pAb (A2027)}

Immunofluorescence - RPS27A Rabbit pAb (A2027)

Immunofluorescence analysis of MCF7 cells using RPS27A Rabbit pAb (A2027) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2027? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)