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RRM1 Rabbit mAb (A4259)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RRM1 Rabbit mAb (A4259)

Western blot analysis of extracts of various cell lines, using RRM1 Rabbit mAb (A4259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - RRM1 Rabbit mAb (A4259)

Western blot analysis of extracts of Rat lung, using RRM1 Rabbit mAb (A4259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for RRM1 Rabbit mAb(A4259)

Overview

Product nameRRM1 Rabbit mAb
Catalog No.A4259
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0943
This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 691-792 of human RRM1 (P23921).
SequenceVLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS
Gene ID
Swiss Prot
SynonymsR1; RR1; RIR1
Calculated MW90kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, A549, Raji, Mouse testis, Rat lung
Cellular locationCytoplasm
Customer validation

(293T 、T47D 、Hela 、Jurkat 、MCF-7 、PC-3 、OAW42 、A375 、Molt-4 )

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - RRM1 Rabbit mAb (A4259)}

    Western blot - RRM1 Rabbit mAb (A4259)

    Western blot analysis of extracts of various cell lines, using RRM1 Rabbit mAb (A4259) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Western blot - RRM1 Rabbit mAb (A4259)}

    Western blot - RRM1 Rabbit mAb (A4259)

    Western blot analysis of extracts of Rat lung, using RRM1 Rabbit mAb (A4259) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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