제품 > 항체 > 다중클론항체(pAb)

RSAD2 Rabbit pAb (A8271)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RSAD2 Rabbit pAb (A8271)

Western blot analysis of various lysates using RSAD2 Rabbit pAb (A8271) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - RSAD2 Rabbit pAb (A8271)

Immunofluorescence analysis of U2OS cells using RSAD2 Rabbit pAb (A8271) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRSAD2 Rabbit pAb
Catalog No.A8271
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an interferon-inducible antiviral protein that belongs to the S-adenosyl-L-methionine (SAM) superfamily of enzymes. The protein plays a role in cellular antiviral response and innate immune signaling. Antiviral effects result from inhibition of viral RNA replication, interference in the secretory pathway, binding to viral proteins and dysregulation of cellular lipid metabolism. The protein has been found to inhibit both DNA and RNA viruses, including influenza virus, human immunodeficiency virus (HIV-1) and Zika virus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 118-361 of human RSAD2 (NP_542388.2).
SequenceEKINFSGGEPFLQDRGEYLGKLVRFCKVELRLPSVSIVSNGSLIRERWFQNYGEYLDILAISCDSFDEEVNVLIGRGQGKKNHVENLQKLRRWCRDYRVAFKINSVINRFNVEEDMTEQIKALNPVRWKVFQCLLIEGENCGEDALREAERFVIGDEEFERFLERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCRKGRKDPSKSILDVGVEEAIKFSGFDEKMFLKRGGKYIWSKADLKLDW
Gene ID
Swiss Prot
SynonymsSAND; cig5; vig1; cig33; RSAD2
Calculated MW42kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesPC-3, Mouse spleen, Rat lung, Rat spleen, Rat kidney
Cellular locationCytoplasmic side, Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus, Lipid droplet, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, Peripheral membrane protein
Customer validation

IF(Mus musculus)

WB(Mus musculus, Homo sapiens)

RT-qPCR(Sus scrofa)

Documents

Certificate of Compliance

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    ABclonal:Western blot - RSAD2 Rabbit pAb (A8271)}

    Western blot - RSAD2 Rabbit pAb (A8271)

    Western blot analysis of various lysates using RSAD2 Rabbit pAb (A8271) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - RSAD2 Rabbit pAb (A8271)}

    Immunofluorescence - RSAD2 Rabbit pAb (A8271)

    Immunofluorescence analysis of U2OS cells using RSAD2 Rabbit pAb (A8271) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8271? Please let us know so that we can cite the reference in this datasheet.