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RSPO1 Rabbit pAb (A8289)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RSPO1 Rabbit pAb (A8289)

Western blot analysis of lysates from SKOV3 cells, using RSPO1 Rabbit pAb (A8289) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - RSPO1 Rabbit pAb (A8289)

Western blot analysis of lysates from Rat pancreas, using RSPO1 Rabbit pAb (A8289) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - RSPO1 Rabbit pAb (A8289)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using RSPO1 Rabbit pAb (A8289) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RSPO1 Rabbit pAb (A8289)

Immunofluorescence analysis of A-549 cells using RSPO1 Rabbit pAb (A8289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RSPO1 Rabbit pAb (A8289)

Immunofluorescence analysis of A-549 cells using RSPO1 Rabbit pAb (A8289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRSPO1 Rabbit pAb
Catalog No.A8289
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human RSPO1 (NP_001033722.1).
SequenceDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQ
Gene ID
Swiss Prot
SynonymsRSPO; CRISTIN3; RSPO1
Calculated MW29kDa
Observed MW29kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSKOV3, Rat pancreas
Cellular locationSecreted.
Customer validation

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - RSPO1 Rabbit pAb (A8289)}

    Western blot - RSPO1 Rabbit pAb (A8289)

    Western blot analysis of lysates from SKOV3 cells, using RSPO1 Rabbit pAb (A8289) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - RSPO1 Rabbit pAb (A8289)}

    Western blot - RSPO1 Rabbit pAb (A8289)

    Western blot analysis of lysates from Rat pancreas, using RSPO1 Rabbit pAb (A8289) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - RSPO1 Rabbit pAb (A8289)}

    Immunohistochemistry - RSPO1 Rabbit pAb (A8289)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using RSPO1 Rabbit pAb (A8289) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - RSPO1 Rabbit pAb (A8289)}

    Immunofluorescence - RSPO1 Rabbit pAb (A8289)

    Immunofluorescence analysis of A-549 cells using RSPO1 Rabbit pAb (A8289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RSPO1 Rabbit pAb (A8289)}

    Immunofluorescence - RSPO1 Rabbit pAb (A8289)

    Immunofluorescence analysis of A-549 cells using RSPO1 Rabbit pAb (A8289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8289? Please let us know so that we can cite the reference in this datasheet.

    재조합 단백질 (1)

    ELISA 키트 (1)

    Secondary Antibodies (26)