제품 > 항체 > 다중클론항체(pAb)

RUSC1 Rabbit pAb (A15417)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RUSC1 Rabbit pAb (A15417)

Western blot analysis of various lysates using RUSC1 Rabbit pAb (A15417) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - RUSC1 Rabbit pAb (A15417)

Immunofluorescence analysis of paraffin-embedded mouse brain using RUSC1 Rabbit pAb (A15417) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RUSC1 Rabbit pAb (A15417)

Immunofluorescence analysis of paraffin-embedded mouse brain using RUSC1 Rabbit pAb (A15417) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRUSC1 Rabbit pAb
Catalog No.A15417
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable actin binding activity. Involved in protein polyubiquitination. Located in cytosol.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human RUSC1 (NP_055143.2).
SequenceMAEAQSGTGQLQEQKKGLLIAVSVSVDKIISHFGAARNLVQKAQLGDSRLSPDVGHLVLTTLCPALHALVADGLKPFRKDLITGQRRSSPWSVVEASVKPGSSTRSLGTLYSQVSRLAPLSSSRSRFHAFILGLLNTKQLELWFSSLQEDAGLLSLLYLPTGFFSLARGGCPSLSTELLLLLQPLSVLTFHLDLLFEHHHHLPLGPPQAPAPPGPPPALQQTMQAMLHFGGRLAQSLRGTSKEAASDPSDSPNLPTPGSWWEQLTQASRVYASGGTEGFPLSRWAPGRHGTAAEEGAQERPLPTDEMAPGRGLWLGRLFG
Gene ID
Swiss Prot
SynonymsNESCA; RUSC1
Calculated MW96kDa
Observed MW55/100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-549, Mouse brain, Mouse lung, Rat lung
Cellular locationCell junction, Cytoplasm, Cytoplasmic vesicle, Early endosome, Golgi apparatus, Nucleus, cytoskeleton, postsynaptic cell membrane, postsynaptic density, synapse

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - RUSC1 Rabbit pAb (A15417)}

    Western blot - RUSC1 Rabbit pAb (A15417)

    Western blot analysis of various lysates using RUSC1 Rabbit pAb (A15417) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - RUSC1 Rabbit pAb (A15417)}

    Immunofluorescence - RUSC1 Rabbit pAb (A15417)

    Immunofluorescence analysis of paraffin-embedded mouse brain using RUSC1 Rabbit pAb (A15417) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RUSC1 Rabbit pAb (A15417)}

    Immunofluorescence - RUSC1 Rabbit pAb (A15417)

    Immunofluorescence analysis of paraffin-embedded mouse brain using RUSC1 Rabbit pAb (A15417) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)