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RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)

Western blot analysis of various lysates using RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Chromatin Immunoprecipitation - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)

Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN Rabbit mAb antibody (A5180) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameRUVBL1/TIP49A/PONTIN Rabbit mAb
Catalog No.A5180
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1247
This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 350-456 of human RUVBL1/TIP49A/PONTIN (Q9Y265).
SequenceIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK
Gene ID
Swiss Prot
SynonymsRVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52; RUVBL1/TIP49A/PONTIN
Calculated MW50kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-2 OS, A-431, HT-1080, Mouse brain, Mouse spleen, Rat brain
Cellular locationCytoplasm, Membrane, Nucleus, Nucleus matrix, centrosome, cytoskeleton, microtubule organizing center, nucleoplasm.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)}

Western blot - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)

Western blot analysis of various lysates using RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)}

Immunohistochemistry - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Chromatin Immunoprecipitation - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)}

Chromatin Immunoprecipitation - RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180)

Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN Rabbit mAb antibody (A5180) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

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