제품 > 항체 > 다중클론항체(pAb)

RXRG Rabbit pAb (A1877)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RXRG Rabbit pAb (A1877)

Western blot analysis of various lysates using RXRG Rabbit pAb (A1877) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RXRG Rabbit pAb (A1877)

Immunohistochemistry analysis of paraffin-embedded rat spleen using RXRG Rabbit pAb (A1877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RXRG Rabbit pAb (A1877)

Immunohistochemistry analysis of paraffin-embedded mouse testis using RXRG Rabbit pAb (A1877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RXRG Rabbit pAb (A1877)

Immunohistochemistry analysis of paraffin-embedded mouse brain using RXRG Rabbit pAb (A1877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameRXRG Rabbit pAb
Catalog No.A1877
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the antiproliferative effects of retinoic acid (RA). This receptor forms dimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene is expressed at significantly lower levels in non-small cell lung cancer cells. Alternatively spliced transcript variants have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human RXRG (NP_008848.1).
SequenceMYGNYSHFMKFPAGYGGSPGHTGSTSMSPSAALSTGKPMDSHPSYTDTPVSAPRTLSAVGTPLNALGSPYRVITSAMGPPSGALAAPPGINLVAPPSSQLNVVNSVSSSEDIKPLPGLPGIGNMNYPSTSPGSLVKH
Gene ID
Swiss Prot
SynonymsRXRC; NR2B3; RXRgamma; RXR-gamma; RXRG
Calculated MW51kDa
Observed MW61kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, DU145, U-87MG, Mouse brain, Rat brain
Cellular locationNucleus

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ABclonal:Western blot - RXRG Rabbit pAb (A1877)}

Western blot - RXRG Rabbit pAb (A1877)

Western blot analysis of various lysates using RXRG Rabbit pAb (A1877) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - RXRG Rabbit pAb (A1877)}

Immunohistochemistry - RXRG Rabbit pAb (A1877)

Immunohistochemistry analysis of paraffin-embedded rat spleen using RXRG Rabbit pAb (A1877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RXRG Rabbit pAb (A1877)}

Immunohistochemistry - RXRG Rabbit pAb (A1877)

Immunohistochemistry analysis of paraffin-embedded mouse testis using RXRG Rabbit pAb (A1877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RXRG Rabbit pAb (A1877)}

Immunohistochemistry - RXRG Rabbit pAb (A1877)

Immunohistochemistry analysis of paraffin-embedded mouse brain using RXRG Rabbit pAb (A1877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)