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S100A7/Psoriasin Rabbit mAb (A22271)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - S100A7/Psoriasin Rabbit mAb (A22271)

Western blot analysis of SK-BR-3, using S100A7/Psoriasin antibody (A22271) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - S100A7/Psoriasin Rabbit mAb (A22271)

Immunohistochemistry analysis of paraffin-embedded human tonsil using S100A7/Psoriasin Rabbit mAb (A22271) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameS100A7/Psoriasin Rabbit mAb
Catalog No.A22271
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56869
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2-101 of human S100A7/Psoriasin (NP_002954.2).
SequenceSNTQAERSIIGMIDMFHKYTRRDDKIEKPSLLTMMKENFPNFLSACDKKGTNYLADVFEKKDKNEDKKIDFSEFLSLLGDIATDYHKQSHGAAPCSGGSQ
Gene ID
Swiss Prot
SynonymsPSOR1; S100A7c
Calculated MW11kDa
Observed MW12kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSK-BR-3
Cellular locationCytoplasm, Secreted

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ABclonal:Western blot - S100A7/Psoriasin Rabbit mAb (A22271)}

Western blot - S100A7/Psoriasin Rabbit mAb (A22271)

Western blot analysis of SK-BR-3, using S100A7/Psoriasin antibody (A22271) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - S100A7/Psoriasin Rabbit mAb (A22271)}

Immunohistochemistry - S100A7/Psoriasin Rabbit mAb (A22271)

Immunohistochemistry analysis of paraffin-embedded human tonsil using S100A7/Psoriasin Rabbit mAb (A22271) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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