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S100A8 Rabbit pAb (A15315)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - S100A8 Rabbit pAb (A15315)

Western blot analysis of various lysates using S100A8 Rabbit pAb (A15315) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Human liver using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Human skin using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - S100A8 Rabbit pAb (A15315)

Immunofluorescence analysis of paraffin-embedded human skin cancer using S100A8 Rabbit pAb (A15315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - S100A8 Rabbit pAb (A15315)

Confocal immunofluorescence analysis of U-2 OS cells using S100A8 Rabbit pAb (A15315) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameS100A8 Rabbit pAb
Catalog No.A15315
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2).
SequenceMLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE
Gene ID
Swiss Prot
SynonymsP8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100-A8; S100A8
Calculated MW11kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, Mouse lung, Mouse kidney
Cellular locationCell membrane, Cytoplasm, Peripheral membrane protein, Secreted, cytoskeleton.
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - S100A8 Rabbit pAb (A15315)}

Western blot - S100A8 Rabbit pAb (A15315)

Western blot analysis of various lysates using S100A8 Rabbit pAb (A15315) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)}

Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)}

Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Human liver using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)}

Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Human skin using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - S100A8 Rabbit pAb (A15315)}

Immunohistochemistry - S100A8 Rabbit pAb (A15315)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using S100A8 Rabbit pAb (A15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - S100A8 Rabbit pAb (A15315)}

Immunofluorescence - S100A8 Rabbit pAb (A15315)

Immunofluorescence analysis of paraffin-embedded human skin cancer using S100A8 Rabbit pAb (A15315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - S100A8 Rabbit pAb (A15315)}

Immunofluorescence - S100A8 Rabbit pAb (A15315)

Confocal immunofluorescence analysis of U-2 OS cells using S100A8 Rabbit pAb (A15315) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A15315? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

ELISA 키트 (2)

재조합 단백질 (1)

Secondary Antibodies (26)