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S100/S100A1 Rabbit pAb (A15588)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - S100/S100A1 Rabbit pAb (A15588)

Western blot analysis of lysates from MDA-MB435 cells, using S100/S100A1 Rabbit pAb (A15588).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Human brain using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Rat brain using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameS100/S100A1 Rabbit pAb
Catalog No.A15588
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human S100/S100A1 (NP_006262.1).
SequenceMGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS
Gene ID
Swiss Prot
SynonymsS100; S100A; S100-alpha; S100/S100A1
Calculated MW11kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMDA-MB435
Cellular locationCytoplasm.

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ABclonal:Western blot - S100/S100A1 Rabbit pAb (A15588)}

Western blot - S100/S100A1 Rabbit pAb (A15588)

Western blot analysis of lysates from MDA-MB435 cells, using S100/S100A1 Rabbit pAb (A15588).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)}

Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Human brain using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)}

Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)}

Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)}

Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)}

Immunohistochemistry - S100/S100A1 Rabbit pAb (A15588)

Immunohistochemistry analysis of paraffin-embedded Rat brain using S100/S100A1 Rabbit pAb (A15588) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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