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SAA3 Rabbit pAb (A11948)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SAA3 Rabbit pAb (A11948)

Western blot analysis of various lysates using SAA3 Rabbit pAb (A11948) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.

Overview

Product nameSAA3 Rabbit pAb
Catalog No.A11948
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables Toll-like receptor 4 binding activity and chemoattractant activity. Acts upstream of or within several processes, including I-kappaB phosphorylation; cellular response to interleukin-1; and response to stilbenoid. Located in extracellular space. Is expressed in cerebellum; ileum; liver; reproductive system; and thymus. Orthologous to several human genes including SAA2 (serum amyloid A2).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-123 of mouse SAA3P (NP_035445.1).
SequenceSDKYFHARGNYDAARRGPGGAWAAKVISDAREAVQKFTGHGAEDSRADQFANEWGRSGKDPNHFRPAGLPKRY
Gene ID
Swiss Prot
Synonymsl7R3; Saa-3; SAA3
Calculated MW13kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman serum, LO2, Mouse liver, Mouse thymus, Mouse lung, Rat liver
Cellular location
Customer validation

WB(Mus musculus)

Documents

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    ABclonal:Western blot - SAA3 Rabbit pAb (A11948)}

    Western blot - SAA3 Rabbit pAb (A11948)

    Western blot analysis of various lysates using SAA3 Rabbit pAb (A11948) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5min.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A11948? Please let us know so that we can cite the reference in this datasheet.