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SARNP Rabbit pAb (A13701)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - SARNP Rabbit pAb (A13701)

Western blot analysis of various lysates using SARNP Rabbit pAb (A13701) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - SARNP Rabbit pAb (A13701)

Immunohistochemistry analysis of paraffin-embedded Human colon using SARNP Rabbit pAb (A13701) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SARNP Rabbit pAb (A13701)

Immunofluorescence analysis of U-2 OS cells using SARNP Rabbit pAb (A13701) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSARNP Rabbit pAb
Catalog No.A13701
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SARNP (NP_149073.1).
SequenceMATETVELHKLKLAELKQECLARGLETKGIKQDLIHRLQAYLEEHAEEEANEEDVLGDETEEEETKPIELPVKEEEPPEKTVDVAAEKKVVKITSEIPQTERMQKRAERFNVPVSLESKKAARAARFGISSVPTKGLSSDNKPMVNLDKLKERAQRFGLNVSSISRKSEDDEKLKKRKERFGIVTSSAGTGTTEDTEAKKRKRAERFGIA
Gene ID
Swiss Prot
SynonymsHCC1; THO1; CIP29; HSPC316; SARNP
Calculated MW24kDa
Observed MW29kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HepG2, BxPC-3, U-87MG, MCF7
Cellular locationNucleus, Nucleus speckle

Documents

Certificate of Compliance

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    ABclonal:Western blot - SARNP Rabbit pAb (A13701)}

    Western blot - SARNP Rabbit pAb (A13701)

    Western blot analysis of various lysates using SARNP Rabbit pAb (A13701) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - SARNP Rabbit pAb (A13701)}

    Immunohistochemistry - SARNP Rabbit pAb (A13701)

    Immunohistochemistry analysis of paraffin-embedded Human colon using SARNP Rabbit pAb (A13701) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - SARNP Rabbit pAb (A13701)}

    Immunofluorescence - SARNP Rabbit pAb (A13701)

    Immunofluorescence analysis of U-2 OS cells using SARNP Rabbit pAb (A13701) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.