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SATB2 Rabbit mAb (A19837)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SATB2 Rabbit mAb (A19837)

Western blot analysis of lysates from Rat brain, using SATB2 Rabbit mAb (A19837) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - SATB2 Rabbit mAb (A19837)

Immunohistochemistry analysis of paraffin-embedded Human appendix using SATB2 Rabbit mAb (A19837) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SATB2 Rabbit mAb (A19837)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SATB2 Rabbit mAb (A19837) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SATB2 Rabbit mAb (A19837)

Immunohistochemistry analysis of paraffin-embedded Human colon using SATB2 Rabbit mAb (A19837) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunoprecipitation - SATB2 Rabbit mAb (A19837)

Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg SATB2 antibody (A19837). Western blot was performed from the immunoprecipitate using SATB2 antibody (A19837) at a dilution of 1:1000.

Overview

Product nameSATB2 Rabbit mAb
Catalog No.A19837
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2363
This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and cognitive disability. Alternate splicing results in multiple transcript variants that encode the same protein.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human SATB2 (Q9UPW6).
SequenceSMISSIVNSTYYANVSATKCQEFGRWYKKYKKIKVERVERENLSDYCVLGQRPMHLPNMNQLASLGKTNEQSPHSQIHHSTPIRNQVPALQPIMSPGLLSP
Gene ID
Swiss Prot
SynonymsGLSS; DEL2Q32Q33; C2DELq32q33; SATB2
Calculated MW83kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesRat brain
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SATB2 Rabbit mAb (A19837)}

    Western blot - SATB2 Rabbit mAb (A19837)

    Western blot analysis of lysates from Rat brain, using SATB2 Rabbit mAb (A19837) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - SATB2 Rabbit mAb (A19837)}

    Immunohistochemistry - SATB2 Rabbit mAb (A19837)

    Immunohistochemistry analysis of paraffin-embedded Human appendix using SATB2 Rabbit mAb (A19837) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SATB2 Rabbit mAb (A19837)}

    Immunohistochemistry - SATB2 Rabbit mAb (A19837)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SATB2 Rabbit mAb (A19837) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SATB2 Rabbit mAb (A19837)}

    Immunohistochemistry - SATB2 Rabbit mAb (A19837)

    Immunohistochemistry analysis of paraffin-embedded Human colon using SATB2 Rabbit mAb (A19837) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunoprecipitation - SATB2 Rabbit mAb (A19837)}

    Immunoprecipitation - SATB2 Rabbit mAb (A19837)

    Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg SATB2 antibody (A19837). Western blot was performed from the immunoprecipitate using SATB2 antibody (A19837) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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