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SCAMP1 Rabbit pAb (A9092)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SCAMP1 Rabbit pAb (A9092)

Western blot analysis of extracts of various cell lines, using SCAMP1 antibody (A9092) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.

Overview

Product nameSCAMP1 Rabbit pAb
Catalog No.A9092
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product belongs to the SCAMP family of proteins, which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that these protein family members may function at the same site during vesicular transport rather than in separate pathways. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SCAMP1 (NP_004857.4).
SequenceMSDFDSNPFADPDLNNPFKDPSVTQVTRNVPPGLDEYNPFSDSRTPPPGGVKMPNVPNTQPAIMKPTEEHPAYTQIAKEHALAQAELLKRQEELERKAAELDRREREMQNLSQHGRKNNWPPLPSNFPVGPCFYQDFSVDIPVEFQKTVK
Gene ID
Swiss Prot
SynonymsSCAMP; SCAMP37
Calculated MW38kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, MCF7, 22Rv1, HT-1080, Mouse brain, Rat brain
Cellular locationGolgi apparatus, Multi-pass membrane protein, Recycling endosome membrane, trans-Golgi network membrane

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    ABclonal:Western blot - SCAMP1 Rabbit pAb (A9092)}

    Western blot - SCAMP1 Rabbit pAb (A9092)

    Western blot analysis of extracts of various cell lines, using SCAMP1 antibody (A9092) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)