제품 > 항체 > 다중클론항체(pAb)

SCN3B Rabbit pAb (A10316)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - SCN3B Rabbit pAb (A10316)

Western blot analysis of Rat brain, using SCN3B Rabbit pAb (A10316) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - SCN3B Rabbit pAb (A10316)

Immunofluorescence analysis of Neuro-2a cells using SCN3B Rabbit pAb (A10316) at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameSCN3B Rabbit pAb
Catalog No.A10316
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-160 of human SCN3B (NP_060870.1).
SequenceFPVCVEVPSETEAVQGNPMKLRCISCMKREEVEATTVVEWFYRPEGGKDFLIYEYRNGHQEVESPFQGRLQWNGSKDLQDVSITVLNVTLNDSGLYTCNVSREFEFEAHRPFVKTTRLIPLRVTEEAGEDFTSVVSEI
Gene ID
Swiss Prot
SynonymsSCNB3; ATFB16; BRGDA7; HSA243396
Calculated MW25kDa
Observed MW30kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:8000
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationMembrane, Single-pass type I membrane protein

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ABclonal:Western blot - SCN3B Rabbit pAb (A10316)}

Western blot - SCN3B Rabbit pAb (A10316)

Western blot analysis of Rat brain, using SCN3B Rabbit pAb (A10316) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - SCN3B Rabbit pAb (A10316)}

Immunofluorescence - SCN3B Rabbit pAb (A10316)

Immunofluorescence analysis of Neuro-2a cells using SCN3B Rabbit pAb (A10316) at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.